Review

p eif4e (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc p eif4e
P Eif4e, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+eif4e/pm41807847-737-23-25
Average 86 stars, based on 1 article reviews

p eif4e - by Bioz Stars, 2026-10

86/100 stars

Images

Western Blot:

Article Title: Remodelling of the translatome controls diet and its impact on tumorigenesis.
Article Snippet: Fasting is associated with a range of health benefits.. How fasting signals elicit changes in the proteome to establish metabolic programmes remains poorly understood.. Here we show that hepatocytes selectively remodel the translatome while global translation is paradoxically downregulated during fasting.

Article Title: Regulation of eIF4E activity for migraine therapy
Article Snippet: .. For western blotting experiments, p-eIF4E (Cell Signaling #9741S) and total-eIF4E (Cell Signaling #9742S) antibodies were used for primary incubation. ..

Article Title: Parthenolide Inhibits Synthesis and Promotes Degradation of Programmed Cell Death Ligand 1 and Enhances T Cell Tumor-Killing Activity.
Article Snippet: Parthenolide is a germacrane sesquiterpene lactone separated from the traditional medicinal plant feverfew.. Previous studies have shown that parthenolide possesses many pharmacological activities, involving anti-inflammatory and anticancer activities.. However, the antitumor mechanism of parthenolide has not been fully elucidated.

FLAG-tag:

other:

Article Title: Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor
Article Snippet: p-eIF4E , Cell Signaling Technology , #9741 , 1:1000.

Incubation:

Article Title: Persistent interferon signaling causes sensory neuron plasticity and pain before and during arthritis.
Article Snippet: .. Membranes were blocked with 5% BSA in 0.1% Tween-20 (TBST) for 1 h at room temperature and then incubated with primary antibody to p-eIF4E (Ser209, Cell Signaling Technology) for 2 days at 4 °C. .. Membranes were washed in TBST three times for 15 min each at room temperature on a shaker and incubated with polyclonal secondary antibody conjugated with horseradish peroxidase (HRP) in 5% BSA (Dako) for 1 h at room temperature.

Ubiquitin Proteomics:

Similar Products

p eif4e (Cell Signaling Technology Inc)

p eif4e - by Bioz Stars, 2026-10

86/100 stars

Buy from Supplier

p erk1 2 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc p erk1 2
P Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+eif4e/eIF4E+Antibody/pm41688788-175-25-26
Average 96 stars, based on 1 article reviews

p erk1 2 - by Bioz Stars, 2026-10

96/100 stars

Buy from Supplier

rabbit anti phospho eif4e p eif4e ser209 polyclonal antibody (Cell Signaling Technology Inc)

Cell Signaling Technology Inc rabbit anti phospho eif4e p eif4e ser209 polyclonal antibody

NNV coat protein translation is initiated by binding of <t>p-eIF4E</t> to the 5′-cap of NNV RNA2 in factories. A Left panel: Upon GGNNV infection (MOI = 100), control and infected GB cells were fixed at 0, 12 and 24 hpi, and immunostained with anti-p-eIF4E-BP and anti-RG-M18 for p-eIF4E-BP (green) and coat protein (red), respectively. Right panel: Statistical analysis of relative cell expression of intracellular distribution of p-eIF4E-BP and coat protein ( n = 54 cells). *, P = 0.04; ***, P = 0.0001; ****, P < 0.0001 (two-way ANOVA test). B Left panel: Detection of NNV RNA2 (green) using RNA FISH with anti-sense RNA2 probe followed by immunocytochemical staining with p-eIF4E (red). Right panel: Statistical analysis of relative cell expression of intracellular distribution of p-eIF4E and RNA2 ( n = 54 cells). *, P = 0.02; ****, P < 0.0001 (two-way ANOVA test). C Left panel: Anti-p-eIF4E and anti-RG-M18 for p-eIF4E (green) and coat protein (red) detection, respectively. Right panel: Statistical analysis of relative cell expression of intracellular distribution of p-eIF4E and coat protein ( n = 54 cells). **, P = 0.002; ****, P < 0.0001 (two-way ANOVA test).The nuclei (blue) were stained with DAPI. Scale bar = 20 μm. DAPI, 4′,6-diamidino-2-phenylindole; GB, grouper brain; GGNNV, giant grouper nervous necrosis virus; hpi, hour post infection; MOI, multiplicity of infection; RNA FISH, RNA fluorescence in situ hybridization

Rabbit Anti Phospho Eif4e P Eif4e Ser209 Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+eif4e/Phospho-eIF4E+(Ser209)+Antibody/pmc12557899-64-0-62
Average 96 stars, based on 1 article reviews

rabbit anti phospho eif4e p eif4e ser209 polyclonal antibody - by Bioz Stars, 2026-10

96/100 stars

Buy from Supplier

antibodies against p-eif4e (Cell Signaling Technology Inc)

Cell Signaling Technology Inc antibodies against p-eif4e

Antibodies Against P Eif4e, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+eif4e/anti+eif4e/pm40367705-77-0-12
Average 90 stars, based on 1 article reviews

antibodies against p-eif4e - by Bioz Stars, 2026-10

90/100 stars

Buy from Supplier

p-eukaryotic translation initiation factor 4e (eif4e)-binding protein 1 (4ebp1) from abclonal biotech co antibody (ABclonal Biotechnology)

ABclonal Biotechnology p-eukaryotic translation initiation factor 4e (eif4e)-binding protein 1 (4ebp1) from abclonal biotech co antibody

P Eukaryotic Translation Initiation Factor 4e (Eif4e) Binding Protein 1 (4ebp1) From Abclonal Biotech Co Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+eif4e/eif4e++a19044++antibody/pmc12007094-47-46-70
Average 90 stars, based on 1 article reviews

p-eukaryotic translation initiation factor 4e (eif4e)-binding protein 1 (4ebp1) from abclonal biotech co antibody - by Bioz Stars, 2026-10

90/100 stars

Buy from Supplier

eif4e p 2 sc 9976 santa cruz wb (Santa Cruz Biotechnology)

Santa Cruz Biotechnology eif4e p 2 sc 9976 santa cruz wb

Eif4e P 2 Sc 9976 Santa Cruz Wb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+eif4e/eIF4E+Antibody/pmc11894096__41467_2025_57539_MOESM1_ESM-84-126-129
Average 96 stars, based on 1 article reviews

eif4e p 2 sc 9976 santa cruz wb - by Bioz Stars, 2026-10

96/100 stars

Buy from Supplier

Image Search Results

NNV coat protein translation is initiated by binding of p-eIF4E to the 5′-cap of NNV RNA2 in factories. A Left panel: Upon GGNNV infection (MOI = 100), control and infected GB cells were fixed at 0, 12 and 24 hpi, and immunostained with anti-p-eIF4E-BP and anti-RG-M18 for p-eIF4E-BP (green) and coat protein (red), respectively. Right panel: Statistical analysis of relative cell expression of intracellular distribution of p-eIF4E-BP and coat protein ( n = 54 cells). *, P = 0.04; ***, P = 0.0001; ****, P < 0.0001 (two-way ANOVA test). B Left panel: Detection of NNV RNA2 (green) using RNA FISH with anti-sense RNA2 probe followed by immunocytochemical staining with p-eIF4E (red). Right panel: Statistical analysis of relative cell expression of intracellular distribution of p-eIF4E and RNA2 ( n = 54 cells). *, P = 0.02; ****, P < 0.0001 (two-way ANOVA test). C Left panel: Anti-p-eIF4E and anti-RG-M18 for p-eIF4E (green) and coat protein (red) detection, respectively. Right panel: Statistical analysis of relative cell expression of intracellular distribution of p-eIF4E and coat protein ( n = 54 cells). **, P = 0.002; ****, P < 0.0001 (two-way ANOVA test).The nuclei (blue) were stained with DAPI. Scale bar = 20 μm. DAPI, 4′,6-diamidino-2-phenylindole; GB, grouper brain; GGNNV, giant grouper nervous necrosis virus; hpi, hour post infection; MOI, multiplicity of infection; RNA FISH, RNA fluorescence in situ hybridization

Journal: Virology Journal

Article Title: Translation of nervous necrosis virus involves eIF4E but not RPS6 phosphorylation and viral particle assembly in remodeled microtubule-organizing center

doi: 10.1186/s12985-025-02799-3

Figure Lengend Snippet: NNV coat protein translation is initiated by binding of p-eIF4E to the 5′-cap of NNV RNA2 in factories. A Left panel: Upon GGNNV infection (MOI = 100), control and infected GB cells were fixed at 0, 12 and 24 hpi, and immunostained with anti-p-eIF4E-BP and anti-RG-M18 for p-eIF4E-BP (green) and coat protein (red), respectively. Right panel: Statistical analysis of relative cell expression of intracellular distribution of p-eIF4E-BP and coat protein ( n = 54 cells). *, P = 0.04; ***, P = 0.0001; ****, P < 0.0001 (two-way ANOVA test). B Left panel: Detection of NNV RNA2 (green) using RNA FISH with anti-sense RNA2 probe followed by immunocytochemical staining with p-eIF4E (red). Right panel: Statistical analysis of relative cell expression of intracellular distribution of p-eIF4E and RNA2 ( n = 54 cells). *, P = 0.02; ****, P < 0.0001 (two-way ANOVA test). C Left panel: Anti-p-eIF4E and anti-RG-M18 for p-eIF4E (green) and coat protein (red) detection, respectively. Right panel: Statistical analysis of relative cell expression of intracellular distribution of p-eIF4E and coat protein ( n = 54 cells). **, P = 0.002; ****, P < 0.0001 (two-way ANOVA test).The nuclei (blue) were stained with DAPI. Scale bar = 20 μm. DAPI, 4′,6-diamidino-2-phenylindole; GB, grouper brain; GGNNV, giant grouper nervous necrosis virus; hpi, hour post infection; MOI, multiplicity of infection; RNA FISH, RNA fluorescence in situ hybridization

Article Snippet: Rabbit anti-phospho-eIF4E (p-eIF4E) (Ser209) polyclonal antibody (#9741), rabbit anti-phospho-eIF4E-BP1 (p-eIF4E-BP) (Ser65) monoclonal antibody (D9G1Q) (#13443), rabbit anti-phospho-MNK1 (p-MNK1) (Thr197/202) polyclonal antibody (#2111), rabbit anti-phospho-p44/42 MAPK (p-ERK) (Thr202/204) monoclonal antibody (D13.14.4E) (#4370), rabbit anti-phospho-p38 MAPK (p-p38) (Thr180/Tyr182) monoclonal antibody (D3F9) (#4511), rabbit anti-phospho-S6 ribosomal protein (p-RPS6) (Ser235/236) monoclonal antibody (D57.2.2E) (#4858) and rabbit anti-phospho-p70S6 kinase (p-p70S6K) (Thr389) monoclonal antibody (#9205) were purchased from Cell Signaling Technology.

Techniques: Binding Assay, Infection, Control, Expressing, Staining, Virus, Fluorescence, In Situ Hybridization

Inhibition of MNK1 phosphorylation in GGNNV-infected GB cells reduces p-MNK1 and p-eIF4E production as well as coat protein translation. A GB cells were infected with GGNNV (MOI = 100). Cells were fixed at 0, 12 and 24 hpi and immunostained for p-MNK1 (green) and coat protein (red). B The cytotoxic effect of MNK1 phosphorylation inhibitor, CGP57380 on GB cells was evaluated by MTT assay. GB cells cultured in a 96-well plate were treated with different concentrations of CGP57380 for 24 h. The cell viabilities were detected with a MTT kit. Values are presented as mean ± SD ( n = 3). C Immunocytochemical staining of p-MNK1 (green) and coat protein (red) in DMSO treated (control) GGNNV-infected GB cells. D Immunocytochemical staining of p-MNK1 (green) and coat protein (red) in 10 µM CGP57380 treated GGNNV-infected GB cells. E Relative coat protein expressions (%) (co-stained with p-MNK1) in DMSO and CGP57380 treated cells were analyzed at 6, 12, 18 and 24 hpi ( n = 54 to 66 cells). F Relative fluorescence intensity of p-MNK1 in DMSO and CGP57380 treated GGNNV-infected cells ( n = 50 to 60 cells). G Relative fluorescence intensity of p-eIF4E in DMSO and CGP57380 treated GGNNV-infected cells ( n = 50 to 60 cells). DMSO and inhibitor treated GB cells were infected with GGNNV (MOI = 100). The samples were collected and fixed at 0, 6, 12, 18 and 24 hpi and then proceeded with immunocytochemical staining using anti-p-eIF4E and anti-RG-M18 antibodies (image is shown in supplementary Fig. ). The mean ± SD for coat protein, p-MNK1 and p-eIF4E were plotted. ns, not significant; **, P < 0.002; ****, P < 0.0001 (two-way ANOVA test). The nuclei (blue) were stained with DAPI. Scale bar = 20 μm. DAPI, 4′,6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; GB, grouper brain; GGNNV, giant grouper nervous necrosis virus; hpi, hour post infection; MOI, multiplicity of infection; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; SD, standard deviation

Journal: Virology Journal

Article Title: Translation of nervous necrosis virus involves eIF4E but not RPS6 phosphorylation and viral particle assembly in remodeled microtubule-organizing center

doi: 10.1186/s12985-025-02799-3

Figure Lengend Snippet: Inhibition of MNK1 phosphorylation in GGNNV-infected GB cells reduces p-MNK1 and p-eIF4E production as well as coat protein translation. A GB cells were infected with GGNNV (MOI = 100). Cells were fixed at 0, 12 and 24 hpi and immunostained for p-MNK1 (green) and coat protein (red). B The cytotoxic effect of MNK1 phosphorylation inhibitor, CGP57380 on GB cells was evaluated by MTT assay. GB cells cultured in a 96-well plate were treated with different concentrations of CGP57380 for 24 h. The cell viabilities were detected with a MTT kit. Values are presented as mean ± SD ( n = 3). C Immunocytochemical staining of p-MNK1 (green) and coat protein (red) in DMSO treated (control) GGNNV-infected GB cells. D Immunocytochemical staining of p-MNK1 (green) and coat protein (red) in 10 µM CGP57380 treated GGNNV-infected GB cells. E Relative coat protein expressions (%) (co-stained with p-MNK1) in DMSO and CGP57380 treated cells were analyzed at 6, 12, 18 and 24 hpi ( n = 54 to 66 cells). F Relative fluorescence intensity of p-MNK1 in DMSO and CGP57380 treated GGNNV-infected cells ( n = 50 to 60 cells). G Relative fluorescence intensity of p-eIF4E in DMSO and CGP57380 treated GGNNV-infected cells ( n = 50 to 60 cells). DMSO and inhibitor treated GB cells were infected with GGNNV (MOI = 100). The samples were collected and fixed at 0, 6, 12, 18 and 24 hpi and then proceeded with immunocytochemical staining using anti-p-eIF4E and anti-RG-M18 antibodies (image is shown in supplementary Fig. ). The mean ± SD for coat protein, p-MNK1 and p-eIF4E were plotted. ns, not significant; **, P < 0.002; ****, P < 0.0001 (two-way ANOVA test). The nuclei (blue) were stained with DAPI. Scale bar = 20 μm. DAPI, 4′,6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; GB, grouper brain; GGNNV, giant grouper nervous necrosis virus; hpi, hour post infection; MOI, multiplicity of infection; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; SD, standard deviation

Techniques: Inhibition, Phospho-proteomics, Infection, MTT Assay, Cell Culture, Staining, Control, Fluorescence, Virus, Standard Deviation

Schematic illustration of how NNV hijacks host machinery for virus protein synthesis and particle assembly. After several rounds of RNA replication/transcription near the mitochondria outer membrane, NNV RNAs are transported from mitochondrial spherules (site of replication) to translation factories. These factories are fused together and later concentrated in perinuclear area at a reorganized MTOC. The resulting viral factories (VFs) are architecturally supported by cytoskeleton proteins. Nuclear movement and reshaping may occur due to LINC complexes connecting nucleus to cytoskeletal elements. The VFs and remodeled MTOC act as a microenvironment to divert host proteins like p-p38, p-ERK, p-MNK1, p-eIF4E, p-eIF4E-BP, RPS6 and other translation factors important for NNV coat protein translation. Outside the VFs and remodeled MTOC, NNV downregulates p-p70S6k/p-RPS6 pathway which phosphorylates RPS6 crucial for host translation. Moreover, NNV inhibits host translation by inducing translocalization and sequestration of PABP in nucleus. These events are followed by degradation of PABP via the 26 S proteasome system . LINC, linker of nucleoskeleton and cytoskeleton; MTOC, microtubule-organizing center; PABP, poly(A) binding protein; VF, Viral Factory. Image created with Biorendor.com

Journal: Virology Journal

Article Title: Translation of nervous necrosis virus involves eIF4E but not RPS6 phosphorylation and viral particle assembly in remodeled microtubule-organizing center

doi: 10.1186/s12985-025-02799-3

Figure Lengend Snippet: Schematic illustration of how NNV hijacks host machinery for virus protein synthesis and particle assembly. After several rounds of RNA replication/transcription near the mitochondria outer membrane, NNV RNAs are transported from mitochondrial spherules (site of replication) to translation factories. These factories are fused together and later concentrated in perinuclear area at a reorganized MTOC. The resulting viral factories (VFs) are architecturally supported by cytoskeleton proteins. Nuclear movement and reshaping may occur due to LINC complexes connecting nucleus to cytoskeletal elements. The VFs and remodeled MTOC act as a microenvironment to divert host proteins like p-p38, p-ERK, p-MNK1, p-eIF4E, p-eIF4E-BP, RPS6 and other translation factors important for NNV coat protein translation. Outside the VFs and remodeled MTOC, NNV downregulates p-p70S6k/p-RPS6 pathway which phosphorylates RPS6 crucial for host translation. Moreover, NNV inhibits host translation by inducing translocalization and sequestration of PABP in nucleus. These events are followed by degradation of PABP via the 26 S proteasome system . LINC, linker of nucleoskeleton and cytoskeleton; MTOC, microtubule-organizing center; PABP, poly(A) binding protein; VF, Viral Factory. Image created with Biorendor.com

Techniques: Virus, Membrane, Binding Assay

Review

Structured Review

Images

Related Articles

Western Blot:

FLAG-tag:

other:

Incubation:

Ubiquitin Proteomics:

Similar Products

Image Search Results